Peptides were purchased from Genosphere, Sigma Aldrich and Bachem companies. All the peptides were chemically synthesised. Aldrich and Bachem supplied the peptides as their Zwitterionic form, Genosphere synthesised the peptides as tri-fluoro acetic salt TFA salts.
The peptides were of purity higher than 98% and were used without any further purification.
All peptides solutions were prepared at 0.001M concentration in milliQ water, Hydrochloric acid HCl 0.002M in order to have relatively the same initial pH of 2.6 value
Tripeptides Glu, Gly, His
The UV Vis Spectra Measurement Properties are:
Wavelength Range (nm.): 300.00 to 800.00
Scan Speed: Medium
Sampling Interval: 0.5
Auto Sampling Interval: Disabled
Scan Mode: Single
Instrument Type: UV-2400PC Series
Measuring Mode: Absorbance
Slit Width: 2.0 nm
Light Source Change Wavelength: 360.0 nm
S/R Exchange: Normal