A variety of cancer cell lines have been infected with a construct that produces a fluorescent or bioluminescent tag. The construct is provided by the Memorial Sloan-Kettering Cancer Centre, New York. Animal models are propagated with the cancer cells. A range of mice and rat animal models are used, including where the cancer cells are introduced through subcutaneous, intratibial and intravenous implantation. Multi-modal in vivo imaging of the animal models using an IVIS imaging system is undertaken at multiple stages. The two-dimensional images show the fluorescent and luciferase tagged cancer cells, and allow monitoring of the tumour growth. X-ray microtomography images reveal the effect of the tumour on the bone and skeleton. Data include the images as well as measurements and graphs, taken from the images, of tumour growth and regression and bone architecture before and after treatment.