Demonstration Environment - development

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Images showing a rapid differential staining technique for Fusarium pseudograminearum in cereal tissues during crown rot infections

University of Southern Queensland
Noel Knight (Principal investigator) Prof Mark W. Sutherland (Associated with)
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ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rfr_id=info%3Asid%2FANDS&rft_id=http://eprints.usq.edu.au/29371/&rft.title=Images showing a rapid differential staining technique for Fusarium pseudograminearum in cereal tissues during crown rot infections&rft.identifier=http://eprints.usq.edu.au/29371/&rft.publisher=University of Southern Queensland&rft.description=Images of wheat and barley tissues colonised by the pathogens Fusarium pseudograminearum, Blumeria graminis f. sp. hordei and Puccinia striiformis were obtained after staining using a novel technique. This new technique was demonstrated to allow differential staining of host and pathogen tissues in leaves and stems. Several staining combinations were assessed. The selected procedure using solophenyl flavine 7GFE and safranin provided high contrast informative images of fungal and plant structures during pathogenesis.&rft.creator=Anonymous&rft.date=2016&rft.relation=http://onlinelibrary.wiley.com/doi/10.1111/j.1365-3059.2011.02462.x/pdf&rft.coverage=159.105459,-9.221084 112.921454,-9.221084 112.921454,-54.777218 159.105459,-54.777218 159.105459,-9.221084&rft_rights=NoLicence&rft_subject=Plant Cell and Molecular Biology&rft_subject=Plant Biology&rft_subject=Biological Sciences&rft_subject=Mycology&rft_subject=Microbiology&rft_subject=Crop and Pasture Protection (pests, Diseases and Weeds)&rft_subject=Crop and Pasture Production&rft_subject=Agricultural and Veterinary Sciences&rft.type=dataset&rft.language=English Go to Data Provider

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Contact Information

noel.knight@usq.edu.au

University of Southern Queensland/ Toowoomba, Qld 4350, Australia

Brief description

Images of wheat and barley tissues colonised by the pathogens Fusarium pseudograminearum, Blumeria graminis f. sp. hordei and Puccinia striiformis were obtained after staining using a novel technique. This new technique was demonstrated to allow differential staining of host and pathogen tissues in leaves and stems. Several staining combinations were assessed. The selected procedure using solophenyl flavine 7GFE and safranin provided high contrast informative images of fungal and plant structures during pathogenesis.

Notes

1 TIFF image plus document

Available:

Data time period: 07 2007 to 09 2011

151.921949,-27.597836 151.937914,-27.597836 151.937914,-27.611451 151.921949,-27.611451 151.921949,-27.597836

151.9299315,-27.6046435

159.105459,-9.221084 112.921454,-9.221084 112.921454,-54.777218 159.105459,-54.777218 159.105459,-9.221084

136.0134565,-31.999151

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